Fermentation process for the production of diphtheria toxin

Details Fermentation process for the production of diphtheria toxin Fermentation process for the production of diphtheria toxin

C - Chemistry, Metallurgy

07

K

C07K 14/34 (2006.01) C12N 1/20 (2006.01) C12N 15/77 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2602143

The present invention relates to a fermentation process comprising a fermentation step of growing a strain of Corynebacterium diphtheria in medium in a fermenter under conditions of agitation sufficient to maintain a homogenous culture and limited aeration such that pO2 within the culture falls to less than 4% for the majority of the fermentation step.

La présente invention concerne un processus de fermentation comprenant une étape de fermentation consistant à mettre une souche de Corynebacterium diphtheria en culture dans un milieu à l'intérieur d'un fermenteur dans des conditions d'agitation suffisantes pour qu'une culture homogène soit conservée et des conditions d'aération limitées pour que le niveau de pO2 dans la culture tombe à moins de 4 % pendant la majeure partie de l'étape de fermentation.

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