Fermentative production and isolation of lactic acid

C - Chemistry – Metallurgy – 12 – P

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C12P 7/56 (2006.01)

Patent

CA 2275758

A method for fermentation of lactic acid from a sugar-containing fermentation liquid in a fermentor by means of lactic acid-forming bacteria, in which whey protein is present or is added as a nutrient substrate for the lactic acid- forming bacteria, wherein at least one protease is added to the fermentor during the fermentation, so that hydrolysis of protein to amino acids takes place simultaneously with the fermentation of sugar into organic acid, and wherein lactic acid resulting from the fermentation is isolated from the fermentation liquid. Ammonia is preferably added to result in the formation of ammonium lactate, and lactic acid is preferably isolated by a process comprising ultra filtration, ion exchange, conventional electrodialysis and electrodialysis with bipolar membranes.

Procédé relatif à la production d'acide lactique par fermentation à partir d'un liquide de fermentation à base de sucre dans un fermenteur, au moyen de bactéries engendrant la formation d'acide lactique, avec présence ou adjonction de protéine de lactosérum en tant que substrat nutritif pour lesdites bactéries, sachant que l'on ajoute dans le fermenteur au moins une protéase en cours de fermentation, de sorte que l'hydrolyse de la protéine en acides aminés ait lieu simultanément avec la fermentation du sucre en acide organique, étant entendu par ailleurs que l'acide lactique issu de la fermentation est isolé du liquide de fermentation. De préférence, on ajoute de l'ammoniac pour engendrer la formation de lactate d'ammonium et on isole l'acide lactique selon un processus incluant l'ultrafiltration, l'échange d'ions, l'électrodialyse classique et l'électrodialyse par membranes bipolaires.

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