Fermentative production of fine chemicals

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 7/46 (2006.01) C12P 13/02 (2006.01) C12P 13/08 (2006.01) C12P 13/10 (2006.01) C12P 13/12 (2006.01) C12P 13/14 (2006.01) C12P 13/24 (2006.01) C12P 19/02 (2006.01) C12P 19/14 (2006.01) C12P 19/20 (2006.01) C12P 25/00 (2006.01)

Patent

CA 2566475

The invention relates to a method for the production of at least one microbial metabolic product having at least 3 C atoms or at least 2 C atoms and at least one N atom by means of sugar-based microbial fermentation. The inventive method involves the following steps: a) producing a sugar-containing liquid medium having a monosaccharide content of more than 20 weight percent from a starch source, wherein the sugar-containing liquid medium also comprises nonstarch-containing solid components of the starch source; b) fermenting the sugar-containing liquid medium for the production of the metabolic product(s) and c) separating or isolating at least one metabolic product from the fermentation broth, wherein the microorganism strain producing the desired metabolic product(s) is cultivated with the sugar-containing liquid medium, which is obtained by: a1) milling the starch source and a2) liquefying the ground material in an aqueous fluid in the presence of at least one starch liquefying enzyme and subsequently saccharifyng the resulting liquid using at least one saccharifying enzyme, wherein at least part of the ground material is liquefied into an aqueous liquid by continuous or discontinuous addition.

Procédé de production d'au moins un métabolite microbien ayant au moins 3 atomes de C ou au moins 2 atomes de C et au moins un atome de N par fermentation microbienne à base de sucre. Ledit procédé consiste (a) à produire un milieu liquide saccharifère ayant une teneur en monosaccharide supérieure à 20 % en poids à partir d'une source d'amidon, ledit milieu liquide saccharifère comprenant également des constituants solides ne contenant pas d'amidon et provenant de la source d'amidon, (b) à faire fermenter le milieu liquide saccharifère en vue de la production du (des) métabolite(s) et (c) à séparer ou à isoler au moins un métabolite du bouillon de fermentation. Selon ledit procédé, une souche de micro-organismes produisant le (les) métabolite(s) souhaité(s) est cultivée avec le milieu liquide saccharifère que l'on obtient (a1) en moulant la source d'amidon et (a2) en liquéfiant la matière moulue dans un liquide aqueux en présence d'au moins une enzyme liquéfiant l'amidon, puis en saccharifiant le liquide obtenu à l'aide d'au moins une enzyme de saccharification, au moins une quantité partielle de la matière moulue étant liquéfiée par ajout contenu et ponctuel de ladite matière au liquide aqueux.

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