Fermentative production of organic compounds using...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/14 (2006.01) C12P 13/08 (2006.01) C12P 13/14 (2006.01)

Patent

CA 2628748

The invention relates to a method for producing at least one organic compound comprising at least 3 C-atoms or at least 2 C-atoms and at least 1 N-atom by fermentation. Said method comprises the following steps: a1) a starch source is ground in order to obtain a grinding material which contains at least one part of the non-starch containing solid components of the starch source; a2) the grinding material is suspended in an aqueous liquid and said grinding material contained in the aqueous liquid is liquefied in the presence of at least one enzyme which liquefies starch, an aqueous substance which contains dextrin (1), which contains at least one part of the non-starch containing solid components of the starch source, is obtained; and b) the aqueous substance which contains dextrin (1) is used in fermentation for cultivating a micro-organism which is capable of over-producing the organic compounds; enzymes, which hydrolyse the dextrin into monosaccharides, are not added or are added in an amount of less than 0.001 wt. %, in relation to the total weight of the used starch source.

La présente invention concerne un procédé pour préparer au moins un composé organique comprenant au moins 3 atomes de C ou au moins 2 atomes de C et au moins 1 atome de N, par fermentation, le procédé comprenant les opérations suivantes : a1) broyage d'une source d'amidon, pour obtenir une matière moulue qui contient au moins une fraction des composantes solides dépourvues d'amidon, de la source d'amidon ; a2) mise en suspension de la matière moulue dans un liquide aqueux, et liquéfaction de la matière moulue contenue dans le liquide aqueux, en la présence d'au moins une enzyme de liquéfaction d'amidon, pour obtenir une substance aqueuse contenant de la dextrine (1) qui contient au moins une fraction des composantes solides dépourvues d'amidon, de la source d'amidon ; et b) utilisation de la substance aqueuse contenant de la dextrine (1) pour une fermentation destinée à la culture d'un micro-organisme qui est capable de réaliser la surproduction du composé organique ; des enzymes qui hydrolysent des dextrines en monosaccharides, n'étant pas ajoutées, ou étant ajoutées en une quantité limitée inférieure à 0,001 % en poids rapportés au poids total de la source d'amidon employée.

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