Fermentive production of four carbon alcohols

Details Fermentive production of four carbon alcohols Fermentive production of four carbon alcohols

C - Chemistry, Metallurgy

12

N

C12N 1/00 (2006.01) C12N 1/21 (2006.01) C12N 1/32 (2006.01) C12P 7/16 (2006.01)

Patent

CA 2722943

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2--butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2- butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. Specifically disclosed herein are the use of coenzyme B12-independent butanediol dehydratases that catalyzes the substrate to product conversion of 2,3-butanediol to 2- butanone in the process of producing 2-butanol and 2-butanone.

L'invention concerne des procédés de production par fermentation de quatre alcools carbonés. Spécifiquement, du butanol, de préférence du 2-butanol, est produit par la croissance fermentative d'une bactérie recombinée exprimant une voie de biosynthèse de 2-butanol. Les micro-organismes recombinés et les procédés de l'invention peuvent également être adaptés à la production de 2-butanone, un intermédiaire dans la voie de biosynthèse du 2-butanol décrite ici. Spécifiquement, l'invention concerne l'utilisation de la coenzyme butanediol déshydratase indépendante de B12 qui catalyse la conversion du substrat en produit de 2,3-butanediol en 2-butanone dans le processus de production de 2-butanol et de 2-butanone.

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