Ferulic acid decarboxylase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/60 (2006.01) A23L 1/23 (2006.01) C12G 3/00 (2006.01) C12G 3/02 (2006.01) C12G 3/06 (2006.01) C12N 1/19 (2006.01) C12N 9/88 (2006.01) C12P 7/22 (2006.01) C12P 7/24 (2006.01) C12P 7/42 (2006.01)

Patent

CA 2228816

The present invention relates to a protein having the amino acid sequence represented by SEQ ID NO: 1, or a protein having ferulic acid decarboxylase activity and having an amino acid sequence wherein one or more amino acid residues are deleted, substituted or added in the amino acid sequence represented by SEQ ID NO: 1; a gene encoding said protein; a recombinant vector comprising said gene; a transformant carrying said recombinant vector; a process for producing 4-vinylguaiacol, vanillin or vanillic acid, or a distilled liquor, wherein an enzyme source having ferulic acid decarboxylase activity which is derived from said transformant is used; and a process for producing a distilled liquor, wherein yeast having an enhanced ferulic acid decarboxylase activity is used.

Le présent brevet porte sur une protéine possédant la séquence d'acide aminée correspondant à SEQ ID NO:1, ou une protéine possédant une acide férulique-décarboxylase et une séquence d'acide aminée dans laquelle au moins un résidu d'acide aminé est supprimé, substitué ou ajouté dans la séquence de l'acide aminée correspondant à SEQ ID NO:1; un gène codant pour cette protéine; un vecteur recombinant comprenant ce gène; un transformant portant ce vecteur recombinant; un procédé pour l'obtention du 4-vinyl-quaiacol, de la vanilline ou de l'acide vanilique, ou une liqueur distillée, dans laquelle on utilise une source enzymatique possédant une acide férulique-décarboxylase qui est dérivée du transformant; et un procédé pour l'obtention d'une liqueur distillée, dans laquelle la levure utilisée possède une acide férulique-décarboxylase à activité accrue.

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