Fiber-optic physiological probes

A - Human Necessities – 61 – B

Patent

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26/197, 73/55, 8

A61B 5/00 (2006.01) G01N 21/64 (2006.01) G01N 21/03 (2006.01) G01N 21/77 (2006.01) G01N 21/78 (2006.01) G01N 21/80 (2006.01) G01N 21/85 (2006.01)

Patent

CA 1329017

FIBER-OPTIC PHYSIOLOGICAL PROBES Abstract Fiber-optic sensors suitable for monitoring physiological analyte concentration. An analyte-permeable matrix is disposed in the light path definedby the axial core at one end of an optical fiber segment. The matrix contains anindicator molecule covalently linked to a polymer, preferably methyl methacrylate/methacrylamidopropyltrimethylammonium chloride, N-vinyl- pyrrolidone/p-aminostyrene, methyl methacrylate/hydroxymethyl methacrylate, methyl methacrylate/N-vinylpyrrolidone, or methyl methacrylate/acrylic acid. In representative embodiments, the polymer is approximately 94:6 mole/mole percent of either methyl methacrylate/methacrylamidopropyltrimethylammonium chloride or N-vinylpyrrolidone/p-aminostyrene copolymer. Drift-free performance is obtained with such sensors having analyte-permeable matrices of significantly less than about 70 microns in thickness. The indicator molecule may be covalently linked to the polymer through an aminoarylalkylamine, such as 4-(aminophenyl)-ethylamine or 4-(aminophenyl)-propylamine. The indicator molecule may be an absorptive molecule, such as phenol red or carboxynaphthophthalein (hydrogen ion analyte), in which case the indicator molecule may be covalently linked to the polymer through either an azo-amide or an amidyl-amide linkage. The indicator molecule may be a luminescent molecule, such as carboxynaphthofluorescein (hydrogen ion analyte) or an oxygen-quenchableporphyrin derivative. The sensor may have a reflector disposed in the light pathdistal with respect to the optical fiber segment to the analyte-permeable matrix. Suitable reflectors include gold foil or films of titanium dioxide, zinc oxide, or barium sulfate. A pCO2 sensor is configured with a gas-permeable but ion- impermeable membrane encapsulating an analyte-permeable matrix that includes a base having a pKa ranging from about 6.0 to about 7.8. The outer membrane may be a silicone, polycarbonate, or urethane. The base may be an inorganic salt, such as bicarbonate, in which case the analyte-permeable matrix should include an antioxidant. Alternatively, the base may be a polymeric base containing, e.g., 2-vinylpyridine, 4-vinylpyridine, histamine, 1-vinylimidazole, or 4-vinylimadazole. Gas-permeability is afforded to the matrix by a minor component of hydrophilic polymer such as polyethylene glycol. A plurality of thepH, pO2, and PCO2 sensors may be disposed together in substantially coterminal array to make a multi-variable probe, which may also include a thermocouple wire.

606579

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