C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) A61K 35/00 (2006.01) A61K 47/42 (2006.01) A61K 48/00 (2006.01) A61L 24/10 (2006.01) C07K 14/745 (2006.01) C12N 15/11 (2006.01) A61K 35/12 (2006.01)
Patent
CA 2375346
Provided, for example, is a method of transforming a cell comprising the steps of: applying a transformation effective amount of a nucleic acid to the cell; applying a fibrin gel to the cell so as to entrap a transformation effective amount of the nucleic acid; and transforming the cell with the nucleic acid. In one aspect, the nucleic acid is applied in admixture with a fibrin or fibrinogen composition that forms the fibrin gel.
présentée à titre d'exemple, cette technique de transformation cellulaire consiste: à appliquer une quantité efficace d'un acide nucléique de transformation vers une cellule, à appliquer un gel de fibrine à la cellule de façon à piéger une quantité efficace d'acide nucléique de transformation, et à transformer la cellule avec cet acide nucléique. Dans un aspect de cette invention, on applique l'acide nucléique sous forme de mélange avec une composition de fibrine ou fibrinogène qui forme le gel de fibrine.
Bristol-Myers Squibb Company
Osler Hoskin & Harcourt Llp
Vivolution A/s
LandOfFree
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