Field-release mass spectrometry

H - Electricity – 01 – J

Patent

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Details

IPC codes

H01J 49/04 (2006.01) C12Q 1/68 (2006.01) H01J 49/16 (2006.01)

Type

Patent

Patent number

CA 2307549

Description

Methods of releasing and analyzing substrates such as DNA, comprising: a) covalently or ligandly binding substrate to a first electrode via a release group, which release group is cleavable in response to applied energy; b) introducing an electrical field so as to establish a charge potential between the first electrode and a second electrode separated by a vacuum or gas phase from the first electrode, the strength of such field sufficient to bristle said covalently-bound or ligandly-bound substrate; and c) applying sufficient energy directly to the release group to cleave the release group and release the substrate into a vacuum or gas phase.

On décrit des procédés de libération et d'analyse de substrats tels que l'ADN, qui consistent à: a) lier de manière covalencielle ou aux ligands un substrat à une première électrode par l'intermédiaire d'un groupe de libération clivable en réponse à une énergie appliquée; b) introduire un champ électrique de manière à établir entre cette première électrode et une deuxième électrode un potentiel de charge séparé de la première électrode par une phase à dépression ou une phase gazeuse, la force de ce champ étant suffisante pour hérisser ledit substrat à liaison covalencielle ou aux ligands; et c) appliquer une énergie suffisante directement sur le groupe de libération pour le cliver et libérer le substrat à l'intérieur de la phase à dépression ou de la phase gazeuse.

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