C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/90 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2559827
The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.
La présente invention porte sur un procédé visant à accroître l'efficacité d'intégration ciblée d'un polynucléotide vers un site prédéterminé dans le génome d'une cellule fongique filamenteuse avec une préférence pour la recombinaison non homologue (RNH), ce polynucléotide ayant une région homologue avec le site prédéterminé. Le procédé consiste à diriger un processus d'intégration vers la recombinaison homologue (RH). La présente invention porte également sur un champignon filamenteux mutant provenant d'une cellule parent, ce mutant ayant un processus de recombinaison homologue (RH) de haut rendement et/ou un processus de recombinaison non homologue (RNH) de faible rendement et/ou un rapport RNH/RH de rendement réduit comparé au rendement RH et/ou RNH et/ou au rapport RNH/RH de la cellule parent dans les mêmes conditions.
Berg Van Den Marco Alexander
Dekker Petrus Jacobus Theodorus
Dsm Ip Assets B.v.
Smart & Biggar
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