Filamentous fungi functional replicating extrachromosomal...

C - Chemistry – Metallurgy – 12 – N

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195/1.21, 195/1.

C12N 15/00 (2006.01) C12N 15/80 (2006.01)

Patent

CA 1207687

22 FILAMENTOUS FUNGI FUNCTIONAL REPLICATING EXTRACHROMOSOMAL ELEMENT ABSTRACT OF THE DISCLOSURE A functional extrachromosomal element capable of replication in filamentous fungi is provided. The extrachromosomal element employs (1) a mitochondrial replicating element or (2) a lower organism replication sequence recognized by the fungus, in combination with foreign DNA to provide replication, transcription, and translation of foreign regulatory elements and genes. The extrachromosomal element is exemplified by a mitochondrial replicating system from Neurospora. The cell strain E. coli HB101 containing the plasmid pALS-1-1 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39157. The cell strain E. coli HB101 containing the plasmid pALS-2 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39158.

431819

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