Filtration

A - Human Necessities – 61 – L

Patent

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Details

A61L 2/02 (2006.01) C07K 1/34 (2006.01) C07K 14/47 (2006.01) C07K 14/755 (2006.01) C07K 14/76 (2006.01) C07K 14/81 (2006.01) C12N 9/64 (2006.01)

Patent

CA 2192683

The present invention relates to a method of virus-filtering a solution that contains at least one macromolecule, by virtue of the total salt content of the solution lying in the range of from about 0.2 M up to saturation of the solution with the salt concerned. The inventive method reduces the residence time and the extent to which the solution need to be diluted, and optimizes the yield when virus-filtering primarily proteins, polysaccharides and polypeptides. The reduction in virus content is at least as good as with conventional techniques where the total salt content is low. The present invention facilitates virus filtration with the aid of the so-called "dead-end" technique, which affords several process and economic advantages in comparison with the tangential virus-filtering technique normally used. When virus-filtering the plasma protein factor IX, the yield obtained in the virus- filtering stags is increased from about 70% to above 95%, by raising the salt. content of the solution in accordance with the present invention.

La présente invention concerne un procédé de filtration de virus dans une solution contenant au moins une macromolécule, la teneur totale en sel de la solution se situant dans la plage d'environ 0,2 M jusqu'à saturation de la solution. Le procédé décrit permet de réduire le temps de séjour et le degré nécessaire de dilution de la solution, et d'optimiser le rendement lors de la filtration de virus principalement dans des protéines, des polysaccharides et des polypeptides. La réduction de la teneur virale est au moins aussi bonne qu'avec des techniques traditionnelles où la teneur totale en sel est faible. La présente invention facilite la filtration virale à l'aide de la technique dite "en bout", laquelle offre plusieurs avantages techniques et économiques par rapport à la technique de filtration virale tangentielle habituellement utilisée. Lors de la filtration de virus dans le facteur protéique plasmatique IX, le rendement obtenu dans la phase de filtration virale est accru d'environ 70 % à plus de 95 %, par élévation de la teneur en sel de la solution selon la présente invention.

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