Filtration columns for different vessel sizes and use

Details Filtration columns for different vessel sizes and use Filtration columns for different vessel sizes and use

G - Physics

01

N

G01N 1/40 (2006.01) B01L 3/00 (2006.01) B01L 3/14 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2450866

This relates to filter columns for isolating nucleic acids. The filter column (1) is adapted for stable placement within the upper portion of standard plastic collection tubes of various sizes. The body of the filter column has a number of surfaces to accommodate placement within variously sized collection tubes. The filter column contains nucleic acid-specific filter which can be located at alternate regions within the filter column, providing different filter surface areas and loading volume capacities using the same column body. The filter column has an opening an upper end adapted to be sealed by a cap. A method for recovering nucleic acids using such filter column is also provided.

L'invention concerne des colonnes de filtres conçues pour isoler des acides nucléiques. La colonne de filtre est conçue pour être insérée de façon stable dans la partie supérieure de tubes de prélèvement en plastique standard de différentes dimensions. Le corps de la colonne de filtre compte plusieurs surfaces, ce qui permet de l'insérer dans des tubes de prélèvement de diverses dimensions. La colonne de filtre contient un filtre spécifique des acides nucléiques pouvant se situer dans des zones en alternance à l'intérieur de ladite colonne, fournissant ainsi différentes zones de surfaces de filtres et capacités de volumes de charge utilisant le même corps de colonne. La colonne de filtre présente une ouverture à l'extrémité supérieure que l'on peut fermer à l'aide d'un capuchon. L'invention concerne également un procédé de récupération des acides nucléiques à l'aide d'une telle colonne de filtre.

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