C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12M 1/38 (2006.01) C12M 1/40 (2006.01)
Patent
CA 2121696
Oligonucleotide primers and methods for indentifying strains of bacteria by genomic fingerprinting are described. The methods are applicable to a variety of samples. The tasting procedure includes amplifying the bacterial DNA in the sample to be tested by adding a pair of outwardly-directed primers to the sample. The primers are capable of hybridizing to repetitive DNA sequences in the bacterial DNA and extending outwardly from one hybridizable repetitive sequence to another hybridizable re- petitive sequence. After amplification the extension products are separated by size and the specific strain of bacteria is deter- mined by measuring the pattern of sized extension products. The procedure to identify strains of bacteria by fingerprinting has a variety of uses including: identifying bacteria in infections, agriculture and horticulture plots, bioremediation, food monitoring, production monitoring, quality assurance and quality control.
Koeuth Thearith
Lupski James R.
Versalovic James
Baylor College Of Medicine
Borden Ladner Gervais Llp
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