Fk-506 cytosolic binding protein

Details Fk-506 cytosolic binding protein Fk-506 cytosolic binding protein

C - Chemistry, Metallurgy

07

K

167/37, 150/9, 5

C07K 14/47 (2006.01) C12N 9/90 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2008149

A new homogeneous cytosolic binding (HCB) protein, having a specific binding activity of about 26 µg FK-506 per mg protein and a molecular weight of about 10-12 kilodaltons, reversibly binds the immunosuppressant FK-506 but not cyclosporine A (CSA). The protein is stable to heating at 56 degrees C. for 30 minutes retaining its FK-506 binding affinity, and has the (partial) amino terminal amino acid sequence: H2N-Gly-Val-Gln-Val- Glu-Thr-Ile-Ser-Pro-Gly-Asp-Gly-Arg-Thr-Phe-Pro-Lys- Arg-Gly-Gln-Thr-X-Val-Val-His-Tyr-Thr-Gly-Met-Leu- Glu-Asp-Gly-Lys-Lys-Phe-Asp (wherein X is undefined). The HCB protein is isolated from the -i- cytosol of mammalian tissues, preferably human neoplastic T-cell lines, e.g. Jurkat, and can be used in diagnostic and purification procedures involving FK-506 macrolide type immunosuppressants. The HCB protein also catalyses the cis-traps isomerization of proline-containing peptide bonds.

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