Flat field correction of two-dimensional biochemical assay...

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G01N 21/93 (2006.01) G01N 27/447 (2006.01) G01N 33/483 (2006.01)

Patent

CA 2450385

The optical imaging of two-dimensional solute zone arrays in electrophoresis gels is corrected for nonuniformities in the optical system such as those arising from the light source or from light dispersion underneath the gel. The correction is achieved by the use of a reference plate that responds to a light source uniformly along its length and width by being either uniformly light absorptive or uniformly light transmissive, or by emitting light upon excitation. Thus, any nonuniformities or deviations in the image of the reference plate arise only from nonuniformities or deviations within the optical system. Analogous corrections are made in other two-dimensional assay images, such as microarrays and microtiter plates.

L'imagerie optique d'essais de zones bidimensionnelles d'un soluté dans l'électrophorèse sur gel corrige les irrégularités du système optique telles que celles venant de la source de lumière ou de la dispersion de lumière sous le gel. On effectue la correction au moyen d'une plaque de référence qui réagit à une source de lumière uniformément sur sa longueur et sa largeur en absorbant ou en émettant la lumière uniformément ou en émettant la lumière lors d'une excitation. Donc, toutes les irrégularités ou déviations dans l'image de la plaque de référence ne proviennent que des irrégularités ou déviations à l'intérieur du système optique. Des corrections analogues sont effectuées dans d'autres images bidimensionnelles d'essais tels que des microréseaux et des plaques de microtitrage.

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