Flexible vaccine assembly and vaccine delivery platform

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) A61K 48/00 (2006.01) C07K 14/08 (2006.01) C07K 19/00 (2006.01)

Patent

CA 2486118

Herein described are various methods for making a vaccine that are made of re- assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different encapsidation intermediate such that the reassembled VLP displays more than one peptide or nucleic acid. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.

On décrit diverses méthodes de production de vaccins à partir de pseudo-particules virales recombinantes (VLP). Premièrement, les VLP sont déassemblées en populations intermédiaires d'encapsidation. Chaque population intermédiaire d'encapsidation est soumise, par exemple, à une conjugaison chimique de fragments peptidiques ou nucléiques uniques pour former des populations séparées. Une quantité préétablie d'un ou de plusieurs intermédiaires d'encapsidation différents issus de ces différentes populations est ensuite mélangée et assemblée pour former des VLP intactes entourant un noyau d'acide nucléique et composées de différents intermédiaires d'encapsidation, si bien que les VLP réassemblées présentent plus d'un peptide ou d'un acide nucléique. L'acide nucléique peut fonctionner seul comme squelette ou être mis au point pour exprimer une protéine immunomodulatrice dans une cellule eucaryote.

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