Flow cytometry analysis across optical fiber

G - Physics – 01 – N

Patent

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Details

G01N 21/59 (2006.01) G01N 15/14 (2006.01) G01N 21/25 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2611565

A flow cytometer type apparatus and method for analyzing a fluid, where the fluid is fed through a passageway within an optical fiber and the light is guided by the fiber across the passageway and intersects the fluid therein. The apparatus includes an optical fiber with a passageway traversing it, a channeling system to channel the fluid medium within the passageway, a light source to propagate light within the optical fiber and across the passageway, and a detection system for detecting an intensity of the light exiting the fiber. The method includes channeling the fluid medium through the passageway, propagating light within the optical fiber, and detecting an intensity of the light output. Preferably, the fluid includes small bodies, like bacteria which are analyzed by assessing the detected exiting light intensity.

L'invention concerne un appareil du type cytomètre en flux et un procédé d'analyse d'un fluide, le fluide étant alimenté à travers un passage dans une fibre optique et la lumière étant guidée par la fibre à travers le passage et croisant le fluide. L'appareil comprend une fibre optique traversée par un passage, un système de canalisation destiné à canaliser le milieu liquide dans le passage, une source lumineuse destinée à propager la lumière dans la fibre optique à travers le passage, ainsi qu'un système de détection destiné à détecter une intensité de la lumière sortant de la fibre. Le procédé consiste à canaliser le milieu liquide à travers le passage, à propager la lumière dans la fibre optique, ainsi qu'à détecter une intensité de la sortie lumineuse. De préférence, le fluide comprend de petits corps, comme des bactéries qui sont analysées par évaluation de l'intensité de lumière sortante détectée.

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