Flow cytometry-based systems and methods for detecting microbes

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/02 (2006.01) C12M 1/34 (2006.01) G01N 33/52 (2006.01) G01N 35/08 (2006.01)

Patent

CA 2734321

In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein.

L'invention concerne des procédés et systèmes pour détecter des microbes dans un échantillon. Les procédés sont généralement applicables pour quantifier le nombre de bactéries cibles dans un échantillon, décompté à partir d'une zone de détection d'un histogramme de cytomètre de flux. Les procédés de détection peuvent être utilisés en présence d'autres micro-organismes et d'autres composants microbiens non cibles pour quantifier sélectivement la quantité d'un microbe cible. Les procédés sont avantageux par rapport à ceux déjà existant pour tester des aliments, et une évaluation de diagnostic de leur vitesse, précision et facilité d'utilisation. Divers dispositifs et kits de collecte à piston utiles pour mettre en pratique l'invention sont également décrits.

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