Flow cytometry lysing reagent with leukoprotective agent for...

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150/3, 167/322

G01N 15/00 (2006.01) G01N 15/14 (2006.01) G01N 33/483 (2006.01) G01N 33/50 (2006.01)

Patent

CA 1227407

FLOW CYTOMETRY LYSING REAGENT WITH LEUKOPROTECTIVE AGENT FOR PRODUCING A 3-PART WBC COUNT ABSTRACT Lysing reagents for use in flow cytometry instruments. Lysing reagents suitable for lysing red blood cells are provided thereby permitting 3-part differential counting of white blood cells. The reagents comprise a leukoprotective agent for preserving the lymphocyte cellular integrity during analysis, and buffers adjusted to provide the correct pH environment for optimal lysis and activity. Preferred embodiments will additionally incorporate non-ionic preservatives in very low concentrations.

461404

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