Flow imaging cytometer

Details Flow imaging cytometer Flow imaging cytometer

G - Physics

01

N

G01N 33/48 (2006.01) G01N 15/14 (2006.01) G01N 21/05 (2006.01) G06K 9/20 (2006.01)

Patent

CA 2050761

-20- FLOW IMAGING CYTOMETER ABSTRACT OF THE DISCLOSURE: A flow imaging cytometer, in which an image capturing area for cells traveling in the flow of a specimen solution is constantly monitored to perform cell photography in an efficient manner, is improved so that fluorescent images of cells can be acquired with greater efficiency. Cell particles to be sensed in the specimen are treated with a fluorescent stain, and the specimen solution is irradiated with light from a pulsed light source for exciting fluores- cence, and with infrared light constantly for monitoring the passage of cells through the cytometer. When the monitoring light is made to serve also as the light for exciting fluorescence, the specimen flow is continuously irradiated at all times at a low luminance for the purpose of monitoring. Then, when a cell particle of interest is detected, the cell particle is irradiated with a light pulse of high luminance to obtain a still picture of the fluorescence-emitting cell. At detection of the cell, a source of white light can be actuated to acquire a white- light image of the cell.

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