G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/53 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2076748
A method of detecting a target moiety comprising the steps of (a) providing an antibody (37) specific to the target, (b) satu- rating the antibody's (37's) binding sites with a labelled form of the target (39), (c) flowing a liquid containing the target past the antibody (37), (d) allowing the target to displace the labelled antigen (39), and (e) detecting the displaced, labelled antigen (39). The invention is accomplished by a flow immunosensor: a flowing liquid moves a sample through the immunosensor; a receiving means (13) introduces the sample to the liquid; a flow control means (21, 23) controls the liquid's movement through the immu- nosensor; and exchanger (35) brings the sample into contact with labelled antigens of the target so that any target present dis- places the labelled antigen (39); a detection apparatus (41, 41a) detects any labelled antigen (39) that is released; and a disposal means (45) disposes of or collects the waste coming from the detection apparatus (41, 41a).
Gaber Bruce P.
Kusterbeck Anne W.
Ligler Frances S.
Wemhoff Gregory A.
Smart & Biggar
The Government Of The United States Of America As Represented By The Se Cretary Of The Department Of The Navy
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