Flow-through hybridization assay for oligonucleotide sequences

Details Flow-through hybridization assay for oligonucleotide sequences Flow-through hybridization assay for oligonucleotide sequences

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/543 (2006.01) G01N 33/544 (2006.01)

Patent

CA 2112645

ABSTRACT Flow-through nucleic acid hybridization assays in which ligand- derivatized probes are synthesized using a target nucleic acid sequence as the template. The probe is immobilized on the membrane of a flow-through assay device by binding to a specific binding pair member for the ligand or hybridization to a complementary nucleic acid sequence. The probe on the membrane of a flow-through assay device, indicating the template target sequence was present during probe synthesis, is detected by means of a tracer comprising a specific binding molecule for the ligand.

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