Fluid handling in microfabricated analytical devices

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 3/08 (2006.01) B01D 61/18 (2006.01) B01D 67/00 (2006.01) B01D 71/02 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) B01L 7/00 (2006.01) C12M 1/26 (2006.01) C12M 1/34 (2006.01) C12M 3/00 (2006.01) C12Q 1/68 (2006.01) G01N 1/28 (2006.01) G01N 33/543 (2006.01) G01N 35/00 (2006.01) A61B 17/04 (2006.01) B01F 5/06 (2006.01)

Patent

CA 2134476

Disclosed are devices for analyzing a fluid cell containing sample. The de- vices comprise a solid substrate (14), microfabricated to define at least one sam- ple inlet port (16A) and a mesoscale flow system (20). The mesoscale flow system (20) includes a sample flow channel (20), extending from the inlet port, and a cell handling region for treating cells disposed in fluid communication with the flowchannel. The devices may further include means for inducing flow of cells in the sample through the flow system. In one embodiment, the cell-handling region (22) may comprise a cell lysis means (24) to enable the lysis of cells in the sample, prior to, e.g., the detection of an intracellular component in the cell sample. In another embodiment, the cell handling region may comprise a cell capture re- gion, comprising binding sites which reversibly bind to a specific population ofcells in the cell sample, to permit the isolation of the specific cell population from the sample. The devices can be utilized in a wide range of automated sensitive and rapid tests for the analysis of a fluid cell containing sample.

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