Fluidic extraction of microdissected samples

G - Physics – 01 – N

Patent

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G01N 1/28 (2006.01) B01L 3/00 (2006.01)

Patent

CA 2338246

Systems and methods are described for processing of laser capture microdissection (LCM) samples. A biological sample processing system includes a laminated film sample processing device including a reaction chamber mated with a biological sample carrier to form a fluidic circuit. A multiple step fluidic device includes an LCM transfer film (910) and a surface that is spaced apart from the transfer film so as to define a fluid volume. The reaction buffer can be removed through an exit port (930), or stop junction, in the surface. Advantages of the systems and methods include facilitating subsequent processing reducing the volume of reagents and enhancing economy. For instance, the reaction buffer can be conveniently removed away from the LCM transfer film.

Cette invention a trait à des dispositifs et aux techniques afférentes permettant le traitement d'échantillons de microdissection par capture laser (LCM). Ce système de traitement de prélèvement biologique comprend un dispositif de traitement de prélèvement à film laminé comportant une chambre à réaction connectée à un support de prélèvement biologique afin de constituer un circuit fluidique. Le dispositif fluidique à plusieurs niveaux comporte un film de transfert de LCM et une surface distante du film de transfert de manière à définir un volume fluidique. Il est possible d'enlever le tampon de réaction par un orifice d'évacuation ou un raccordement d'arrêt de sortie, au niveau de la surface. Entre autres avantages, ces dispositifs et techniques facilitent le traitement ultérieur du fait de la réduction de la quantité des réactifs et permettent de réduire les coûts. Le tampon de réaction peut, par exemple, être commodément retiré du film de transfert de LCM.

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