Fluorescein labelled phosphoramidites

Details Fluorescein labelled phosphoramidites Fluorescein labelled phosphoramidites

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C07F 9/6561 (2006.01)

Patent

CA 2050706

Abstract Compounds useful to attach a fluorescein label to an oligonucleotide are disclosed. To create these compounds, oxygen groups on the fluorescein moiety are protected (e.g. with acyl groups). One then links a phosphoramidite to the fluorescein moiety via an amide or thiourea linkage to an active site on the fluorescein ring. The resulting compound can then be directly linked to an oligonucleotide as the oligonucleotide is formed in an automated synthesizer and then deprotected using the same deprotection conditions are used to deprotect the oligonucleotide. Such compounds are, inter alia, useful to create labelled primer for DNA sequencing. \fluor-lp

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