Fluorescence assay apparatus

Details Fluorescence assay apparatus Fluorescence assay apparatus

G - Physics

01

N

G01N 21/01 (2006.01) G01N 21/64 (2006.01) G01N 21/77 (2006.01) G01N 33/52 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2073344

A system for use with a totally internally reflecting fiber-optic fluor- escent assay apparatus (20) overcomes the problems of discriminating against internally generated fluorescence-arising in polymeric fibers. The system includes a disc (50) that can be moved to alternately block and un- block a central colinear conical portion of an input cone of excitation radia- tion directed into an input end (28) of the fiber (30), thereby periodically varying the numerical aperture at the sensing region of the fiber surface. By analyzing the signal values received when that numerical aperture is rela- tively high with respect to those received when that numerical aperture is relatively low, one can determine what portion of the fluorescence at the sensing region (44) on the fiber surface as distinguished from various back- ground fluorescence.

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