Fluorescence-based adp detection system

Details Fluorescence-based adp detection system Fluorescence-based adp detection system

G - Physics

01

N

G01N 21/64 (2006.01)

Patent

CA 2553218

A method and kit for detecting a nucleotide, or differentially detecting one nucleotide in a mixture of at least two nucleotides, in a solution comprising the steps of adding a chelator to the solution and detecting a signal created or altered upon the addition of the chelator using a spectroscopic detection system. A lanthanide may also be added to the solution before the detection of a signal. Preferably, the spectroscopic detection system is a fluorescence- based system.

Méthode et trousse de détection d'un nucléotide, ou de détection différentielle d'un nucléotide dans un mélange d'au moins deux nucléotides dans une solution. On ajoute un agent chélateur à la solution et on détecte un signal généré ou modifié par l'adjonction du chélateur en utilisant un système de détection spectroscopique. On peut également ajouter un lanthanide à la solution avant de détecter le signal. De préférence, le système de détection spectroscopique est un système à base de fluorescence.

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