Fluorescence competitive binding assay for thyroid hormone

Details Fluorescence competitive binding assay for thyroid hormone Fluorescence competitive binding assay for thyroid hormone

G - Physics

01

N

167/43

G01N 33/53 (2006.01) G01N 33/78 (2006.01)

Patent

CA 1106280

ABSTRACT OF THE DISCLOSURE Thyroid hormones such as thyroxine and triiodo- thyronine are assayed in biological fluid samples by a competitive binding analysis in which a fluorescent label is used. Upon formation of com- plexes, the fluorescence of the labelled compound is enhanced, and the hormone can be assayed by measuring the fluorescence of the mixture and com- paring the result with standard data. Fluorescein is the preferred label, The process can be operated in a continuous flow manner.

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