Fluorescence illumination assembly for an imaging apparatus

G - Physics – 01 – N

Patent

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Details

G01N 21/64 (2006.01) G02B 6/38 (2006.01)

Patent

CA 2477269

A fluorescence imaging assembly including an imaging apparatus having an enclosure wall defining a view port into a light-tight imaging compartment thereof. A specimen platform is positioned in the imaging compartment having a support surface facing toward the view port. An illumination assembly includes a light source and a frame disposed in the imaging compartment. The illumination assembly is positioned proximate to and substantially peripherally encircling the view port. A bundle of fiber optic strands extends into the imaging compartment wherein proximal ends of the strands are in optical communication with the light source and distal ends thereof terminate at the frame to emit a conical directional beam of light onto the specimen platform. The distal ends of the fiber optic strands are sufficiently spaced peripherally about the view port such that the plurality of directional beams collectively illuminate the specimen platform in a substantially uniform manner.

L'invention concerne un ensemble d'imagerie fluorescent comprenant un appareil d'imagerie doté d'une paroi d'enveloppe définissant un orifice de visualisation dans un compartiment d'imagerie étanche à la lumière. Une plate-forme d'échantillon est positionnée dans le compartiment d'imagerie doté d'une surface de support opposée à l'orifice de visualisation. Un ensemble d'éclairage comprend une source lumineuse et un cadre disposés dans le compartiment d'imagerie. Ledit ensemble d'éclairage est positionné à proximité de l'orifice de visualisation et l'entoure sensiblement. Un faisceau de brins de fibres optiques s'étend dans le compartiment d'imagerie, les extrémités proximales des brins étant en communication optique avec la source lumineuse et les extrémités distales desdits brins se terminant au niveau du cadre afin d'émettre un faisceau lumineux directionnel conique sur la plate-forme d'échantillon. Les extrémités distales des brins de fibres optiques sont suffisamment écartées à la périphérie, autour l'orifice de visualisation, de sorte que la pluralité de faisceaux directionnels éclairent collectivement la plate-forme d'échantillon de manière sensiblement uniforme.

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