Fluorescence-labeled substrates and their use to analyze...

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/37 (2006.01) C12Q 1/25 (2006.01) C12Q 1/34 (2006.01) C12Q 1/48 (2006.01) C12Q 1/527 (2006.01)

Patent

CA 2220656

The present invention involves a reagent suitable for determining the presence or absence of enzymatic activity in a test sample using flow cytometry, comprising an enzyme substrate immobilized on a solid support. The invention also involves a method of determining the presence or absence of enzymatic activity in a test sample using a fluorophore-labeled enzyme substrate immobilized on a solid support under such conditions as to allow enzymatic digestion of the labeled substrate, washing the digested immobilized substrate, passing the digested immobilized substrate through a flow cytometer, and identifying the amount of net enzyme activities in the test sample based on the measured signals.

L'invention porte sur un réactif permettant de déterminer la présence ou l'absence d'activité enzymatique dans un échantillon d'analyse grâce à la cytométrie de flux, à l'aide d'un substrat d'enzyme fixé sur un support solide. L'invention porte également sur une méthode pour déterminer la présence ou l'absence d'activité enzymatique dans un échantillon d'analyse grâce à un substrat d'enzyme marqué par un groupe fluorescent, fixé sur un support solide dans des conditions permettant la digestion enzymatique du substrat marqué; on lave ensuite ce dernier et on le fait passer dans un cytomètre de flux, pour finalement déterminer l'activité enzymatique nette dans l'échantillon d'analyse à partir des signaux mesurés.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Fluorescence-labeled substrates and their use to analyze... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescence-labeled substrates and their use to analyze..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescence-labeled substrates and their use to analyze... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1862992

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.