Fluorescence-mediated molecular tomography

A - Human Necessities – 61 – B

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A61B 5/00 (2006.01) A61B 6/00 (2006.01) A61K 49/08 (2006.01) G01N 21/00 (2006.01) G01N 21/47 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2428462

System (10) includes a continuous (CW) laser source (12). The laser (12) uses constant intensity light. Two wavelengths obtained from the two lasers can be used for imaging the intrinsic contrast. The optical switch (16) directs light from laser (12) to any one of multiple fibers (18). Fluorescent light collected by multipoint detector array (21) is fed through detector fibers (22) to a two-dimensional emitted fluorescent light array (24). The two- dimensional array (21) transmits the fluorescence signals to a long pass filter (25) and to CCD camera (26) providing an image.

Cette invention concerne un système d'imagerie tomographique moléculaire induite par fluorescence qui est conçue pour détecter une activation de la fluorescence dans le proche infrarouge au niveau de tissus profonds. Le système repose sur l'emploi de sondes moléculaires fluorescentes ciblées ou de sondes moléculaires hautement sensibles à fluorescence activable. De telles sondes ajoutent une spécificité moléculaire et produisent un contraste de fluorescence marqué, ce qui favorise une détection précoce et une évaluation de cibles moléculaires sur des tissus malades, tels que des tissus cancéreux, in vivo. Le nouveau système d'imagerie tomographique autorise une localisation tridimensionnelle dans des tissus profonds et une quantification des sondes moléculaires.

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