Fluorescence polarization assay for macromolecular...

Details Fluorescence polarization assay for macromolecular... Fluorescence polarization assay for macromolecular...

C - Chemistry, Metallurgy

12

Q

150/15.2, 150/3.

C12Q 1/34 (2006.01) C12Q 1/37 (2006.01) C12Q 1/40 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 1279242

ABSTRACT OF THE DISCLOSURE A fluorescence polarization assay for determining the presence and/or amount of macromolecular hydrolases such as polysaccharide hydrolases, amylase, trypsin, proteases and lipases is disclosed. The disclosure provides reagents, processes for making the reagents useful in the assay and processes for conducting the assay. The assay involves the use of a substrate capable of being cleaved by the macromolecular hydrolase being determined, wherein the substrate is coupled to a fluorophore.

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