Fluorescence polarization assays involving polyions

G - Physics – 01 – N

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G01N 33/542 (2006.01) C12Q 1/37 (2006.01) C12Q 1/42 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2373747

Methods, systems, kits for carrying out a wide variety of different assays that comprise providing a first reagent mixture which comprises a first reagent having a fluorescent label. A second reagent is introduced into the first reagent mixture to produce a second reagent mixture, where the second reagent reacts with the first reagent to produce a fluorescently labeled product having a substantially different charge than the first reagent. A polyion is introduced into at least one of the first and second reagent mixtures, and the fluorescent polarization in the second reagent mixture relative to the first reagent mixture is determined, this fluorescent polarization being indicative of the rate or extent of the reaction.

Cette invention concerne des procédés, des systèmes et des kits qui permettent d'effectuer un grand nombre d'analyses, et dans lesquels on utilise un premier mélange réactif comprenant un premier réactif ayant un marqueur fluorescent. Un second réactif est introduit dans le premier mélange réactif de manière à produire un second mélange réactif, le second réactif réagissant avec le premier de manière à produire un produit marqué par fluorescence qui possède une charge essentiellement différente de celle du premier réactif. Un polyion est introduit dans le premier et/ou le second mélanges réactifs, et l'on détermine la polarisation fluorescente dans le second mélange réactif par rapport au premier, cette polarisation indiquant la vitesse ou l'étendue de la réaction.

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