Fluorescence polarization detection of nucleic acids

Details Fluorescence polarization detection of nucleic acids Fluorescence polarization detection of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 19/00 (2006.01) C12M 1/00 (2006.01) C12M 1/34 (2006.01) C12P 19/34 (2006.01) G01N 21/64 (2006.01) G01N 21/78 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2486410

The apparatus and method described herein detect fluorescence polarization (FP) during a nucleic acid reaction such as PCR amplification or isothermal amplification. Fluorescence polarization can be concurrently detected in multiple samples. In addition, multiple different fluorophores can be used for detect different sequences within a sample during the same reaction.

Cette invention concerne un appareil et un procédé permettant de détecter la polarisation de fluorescence lors d'une réaction d'acides nucléiques telle qu'une amplification PCR ou amplification isothermique. La polarisation de fluorescence peut être détectée simultanément dans de multiples échantillons. En outre, de multiples fluorophores différents peuvent être utilisés pour détecter différentes séquences dans un échantillon lors de la même réaction.

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