Fluorescent detection of nonviable cells

Details Fluorescent detection of nonviable cells Fluorescent detection of nonviable cells

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2153257

A method for fluorescent discrimination of cells that are nonviable prior to lysis or fixation techniques using a complementary pair of nucleic acid dyes which competitively bind to nucleic acid but which differ in their fluorescent emission characteristics. An especially preferred pair of dyes is 7-aminoactinomycin D (7-AAD) and actinomycin D (AD).

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