Fluorescent detection of proteins in polyacrylamide gels

Details Fluorescent detection of proteins in polyacrylamide gels Fluorescent detection of proteins in polyacrylamide gels

G - Physics

01

N

G01N 33/68 (2006.01) G01N 27/26 (2006.01)

Patent

CA 2473925

The UV light-induced reaction between the indole moiety of tryptophan (Trp) and haloalkanes permits rapid visualization of protein resolved by polyacrylamide gel electrophoresis (PAGE). The gel incorporates a haloalkane which reacts with Trp and fluoresces under UV light. The haloalkane may be incorporated prior to polymerization of the gel or subsequently.

Selon la présente invention, la réaction induite par UV entre la fraction indole de tryptophane (Trp) et des haloalcanes permet la visualisation rapide de protéines séparées par électrophorèse en gel de polyacrylamide (PAGE). Ledit gel comprend un haloalcane qui réagit avec le Trp et fluoresce sous UV. Ledit haloalcane peut être incorporé préalablement à la polymérisation du gel ou ultérieurement.

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