Fluorescent energy transfer immunoassay

Details Fluorescent energy transfer immunoassay Fluorescent energy transfer immunoassay

A - Human Necessities

61

K

A61K 49/00 (2006.01) G01N 21/64 (2006.01) G01N 33/53 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2087413

ABSTRACT OF THE INVENTION A fluorometric luminescence immunoassay method includes forming a sample by exposing a first immune reaction reactant to a second immune reaction reactant capable of reacting with the first reactant, one of the first and second immune reaction reactants being labelled with a photoluminescent energy transfer donor and the other being labelled with a photoluminescent energy transfer acceptor complementary to the photoluminescent donor. At least the photoluminescent donor has the property of photoluminescence, and the photoluminescent donor and acceptor are chosen so that when the first immune reaction reactant reacts with the second immune reaction reactant, the donor and the acceptor are capable of interacting to produce a detectable luminescence lifetime change. The sample is excited with radiation, and the resulting emission is detected. The apparent luminescent lifetime is then calculated to determine the presence of a reaction product of the first and second immune reaction reactants.

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