G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/542 (2006.01) G01N 21/64 (2006.01) G01N 33/533 (2006.01) G01N 33/543 (2006.01) G01N 33/544 (2006.01)
Patent
CA 2257941
This invention relates to a fluorescence-based immunoassay method for the detection of an analyte, or for the measurement of its concentration in a biological sample. The method is based on the ability of a multivalent analyte to induce aggregation of receptor molecules labeled with a fluorophore, which molecules are anchored to and are freely mobile on a lipid membrane, and thereby cause changes in the fluorescence.
La présente invention concerne une technique de dosage immunologique utilisant les phénomènes de fluorescence pour détecter un analyte ou pour mesurer sa concentration dans un prélèvement biologique. Le procédé repose sur l'aptitude d'un analyte multivalent à induire l'agrégation de molécules réceptrices marquées d'un fluorophore, lesquelles molécules sont ancrées sur une couche lipidique où elles sont librement mobiles, causant de ce fait des modifications de fluorescence.
Keinanen Kari
Laukkanen Marja-Leena
Soderlund Hans
Mccarthy Tetrault Llp
Valtion Teknillinen Tutkimuskeskus
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