Fluorescent immunoassay apparatus and method

Details Fluorescent immunoassay apparatus and method Fluorescent immunoassay apparatus and method

G - Physics

01

N

73/51.5

G01N 21/64 (2006.01) G01N 21/77 (2006.01) G01N 33/53 (2006.01)

Patent

CA 1277506

ABSTRACT An assay method and apparatus employing total inter- nal reflection of excitation radiation at an activated region on the surface of an optically conductive rod or fiber surrounded by a liquid phase of lower index of refraction, so as to excite into fluorescence fluorophores in the liquid present in a thin layer around the rod surface. The induced fluorescence is then gathered and measured. Excitation occurs while flowing a sample of the liquid phase through an enclosure of fixed dimensions bounded in part by the activated region.

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