Fluorescent indicator dyes for calcium working at long...

Details Fluorescent indicator dyes for calcium working at long... Fluorescent indicator dyes for calcium working at long...

C - Chemistry, Metallurgy

09

B

260/282, 260/328

C09B 15/00 (2006.01) C09B 11/24 (2006.01) C09B 57/00 (2006.01) G01N 33/84 (2006.01)

Patent

CA 1332416

FLUORESCENT INDICATOR dyes for CALCIUM WORKING AT LONG WAVELENGTHS ABSTRACT The present invention discloses a new class of calcium specific fluorescent indicator dyes having visible excitation and emission wavelengths. The new fluorescent indicator dyes combine at least one tricyclic chromophore with a tetracarboxylate parent CA2+ chelating compound having the octacoordinate pattern of liganding groups characteristic of BAPTA to give a rhodamine-like or fluorescein-like fluorophore. Binding of calcium2+ increases the fluorescence of the new compounds by up to 40-fold. The caicium2+ dissociation constants are in the range 0.37-2.3 microM, 60 that the new indicators give better resolution of high [CA2+] levels than were previously obtainable with predecessor compounds such as quin-2 or fluo-2. The visible excitation wavelengths of the new compounds are more convenient for fluorescent microscopy and flow cytometry than the UV required by previous indicators.

579196

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