Fluorescent membrane intercalating probes and methods for...

C - Chemistry – Metallurgy – 07 – D

Patent

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C07D 209/04 (2006.01)

Patent

CA 2630046

The invention relates to a family of dyes which fluoresce in the UV-VIS, far red and near infrared wavelengths of the spectrum and possess asymmetric lipophilic alkyl chains. The dyes of the invention are soluble in commercially available membrane staining dyes, are useful as probes for rapidly staining lipophilic structures such as membranes in cells or isolated from cells, and are well-retained therein. Methods of utilizing the dyes to detect stained cells both in vivo and in vitro are also disclosed.

L~invention concerne une famille de colorants émettant une fluorescence aux longueurs d~ondes UV-VIS, infrarouge lointain et infrarouge proche du spectre, et dotés de chaînes alkyles lipophiles asymétriques. Les colorants de l~invention sont solubles dans des colorants du commerce utilisés pour marquer les membranes ; ils s~avèrent utiles en tant que sondes pour structures lipophiles à coloration rapide telles que des membranes dans les cellules ou isolées à partir de cellules, et sont retenus de façon satisfaisante par ces dernières. L~invention concerne également des procédés de mise en AEuvre desdits colorants pour détecter, tant in vivo qu~in vitro, des cellules marquées.

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