Fluorescent membrane intercalating probes and methods for...

C - Chemistry – Metallurgy – 07 – D

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C07D 209/60 (2006.01) A61K 49/00 (2006.01) C07D 209/14 (2006.01) C07D 215/12 (2006.01) C09B 23/08 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2402352

The invention relates to a family of cyanine dyes which fluoresce in the far red and near infra red wavelengths of the spectrum and preferably possess lipophilic side chains. The dyes of the invention are soluble in commercially available membrane staining vehicles, are useful as probes for rapidly staining lipophilic structures such as membranes in cells or isolated from cells, and are well retained therein. Methods of using the dyes to detect stained cells both in vivo and in vitro are also disclosed.

La présente invention concerne une famille de colorants de cyanine qui sont fluorescents dans les longueurs d'ondes du rouge lointain et du proche infrarouge du spectre et possèdent, de préférence, des chaînes latérales lipophiles. Les colorants selon l'invention sont solubles dans les excipients colorants des membrane disponibles dans le commerce et sont utiles comme sondes pour colorer rapidement les structures lipophiles telle que des membranes dans des cellules ou isolées à partir de cellules, et sont bien maintenus dans ces dernières. L'invention a aussi pour objet des procédés permettant d'utiliser des colorants pour détecter des cellules colorées tant in vivo que in vitro.

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