Fluorescent methods for the high-throughput quantification...

Details Fluorescent methods for the high-throughput quantification... Fluorescent methods for the high-throughput quantification...

C - Chemistry, Metallurgy

12

Q

C12Q 1/42 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2524669

Methods for quantifying tartrate-resistant acid phosphotase (TRAP) activity, or determining the distribution of TRAP in a tissue are provided. More specifically, the fluorescent assay of the present invention is useful for identifying or quantifying TRAP in isolated osteoclast cells or osteoclasts differentiated from RAW264.7 monocyte-macrophage cells.

La présente invention concerne des procédés permettant de quantifier l'activité phosphatase acide résistante au tartrate (TRAP), ou de déterminer la répartition de la TRAP dans un tissu. Plus particulièrement, le dosage par fluorescence décrit dans la présente invention est utile pour identifier ou quantifier la TRAP dans des cellules ostéoclastiques isolées ou des ostéoclastes différenciés à partir des macrophages monocytaires RAW264.7.

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