Fluorescent multiplex hpv pcr assays using multiple...

Details Fluorescent multiplex hpv pcr assays using multiple... Fluorescent multiplex hpv pcr assays using multiple...

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/37 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2457888

The present invention relates a fluorescent multiplex PCR assay for detecting the presence of an HPV subtype in a sample using multiple fluorophores to simultaneously detect a plurality of HPV genes of the same HPV subtype. The present invention also relates to primer pairs and probes specific to HPV subtypes for use in the methods of the present invention.

L'invention concerne un dosage PCR multiplex fluorescent permettant de détecter la présence d'un sous-type de virus du papillome humain (HPV) dans un échantillon à l'aide de multiples fluorophores, afin de détecter simultanément une pluralité de gènes de HPV du même sous-type de HPV. Cette invention concerne également des paires d'amorces et des sondes spécifiques de sous-types de HPV destinées à être mises en oeuvre dans les procédés de cette invention.

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