Fluorescent nucleobase conjugates having anionic linkers

Details Fluorescent nucleobase conjugates having anionic linkers Fluorescent nucleobase conjugates having anionic linkers

C - Chemistry, Metallurgy

07

H

C07H 19/20 (2006.01) C07H 19/04 (2006.01) C07H 19/06 (2006.01) C07H 19/10 (2006.01) C07H 19/16 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) C07B 61/00 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2425663

Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.

L'invention concerne des conjugués nucléotide-colorant et des composés connexes, dans lesquels un colorant est lié à une nucléobase directement ou indirectement par un lieur anionique. Le caractère anionique du lieur provient d'une ou de plusieurs fractions anioniques qu'il contient, telles que des groupes phosphate, phosphonate, sulfonate et carboxylate. Lorsque le colorant est un couple donateur/accepteur, le lieur anionique peut être situé entre le donateur et l'accepteur ou entre la nucléobase d'une part et le donateur ou l'accepteur d'autre part. Dans un mode de réalisation, les conjugués présentent des caractéristiques de mobilité électrophorétique renforcées pour le séquençage de fragments, c.-à-d. pour le séquençage didésoxy faisant appel à des terminateurs marqués.

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