Fluorescent oxygen channeling immunoassays

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07D 311/20 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2170873

Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a photoactive indicator precursor molecule to come into close proximity. The photosensitizer generates singlet oxygen which activates the photoactive indicator precursor to generate a photoactive indicator molecule. Upon irradiation with light the photoactive indicator molecule produces light, which is measured. The amount of light produced by the photoactive indicator is related to the amount of analyte in the medium. Compositions, kits, and compounds are also disclosed.

On décrit des procédés permettant de détecter un analyte dans un milieu soupçonné d'en contenir. Un de ces procédés consiste à traiter un tel milieu dans des conditions telles que l'analyte éventuel amène en étroite proximité un photosensibilisateur et une molécule précurseur d'indicateur photoactif. Le photosensibilisateur produit de l'oxygène singulet qui active le précurseur d'indicateur photoactif pour donner une molécule d'indicateur photoactif, laquelle produit, par illumination, une lumière que l'on mesure. La quantité de lumière produite par l'indicateur photoactif est liée à la quantité d'analyte présente dans le milieu. On décrit aussi des compositions, trousses et composés concernant ces procédés.

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