Fluorescent polymer superquenching-based bioassays

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/70 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01) G01N 33/537 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2441279

A chemical composition including a fluorescent polymer and a receptor that is specific for both a target biological agent and a chemical moiety including (a) a recognition element, (b) a tethering element, and (c) a property- altering element is disclosed. Both the fluorescent polymer and the receptor are co-located on a support. When the chemical moiety is bound to the receptor, the property-altering element is sufficiently close to the fluorescent polymer to alter the fluorescence emitted by the polymer. When an analyte sample is introduced, the target biological agent, if present, binds to the receptor, thereby displacing the chemical moiety from the receptor, resulting in an increase of detected fluorescence. Assays for detecting the presence of a target biological agent are also disclosed.

La présente invention concerne une composition chimique comprenant un polymère fluorescent et un récepteur spécifique d'un agent biologique cible, et un fragment chimique incluant (a) un élément de reconnaissance, (b) un élément de retenue, et (c) un élément modificateur des propriétés. Le polymère fluorescent ainsi que le récepteur se trouvent sur un même support. Lorsque le fragment chimique est lié au récepteur, l'élément modificateur des propriétés est assez près du polymère fluorescent pour en modifier la fluorescente. Lorsqu'on introduit un échantillon d'analysat, l'agent biologique cible, s'il est présent, se lie au récepteur, ce qui éloigne le fragment chimique du récepteur, d'où une augmentation de la fluorescence détectée. L'invention concerne également des dosages permettant de détecter la présence d'un agent biologique cible.

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