Fluorescent protein

Details Fluorescent protein Fluorescent protein

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 5/10 (2006.01) C12Q 1/02 (2006.01) G01N 21/78 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2483646

It is intended to provide a fluorescent protein having a novel primary structure originating in an organism other than Aequorea coerulescens. Thus, a fluorescent protein originating inHalcurias sp. L which has the following properties is provided: (1) having a maximum excitation wavelength of 494 nm and a maximum fluorescent wavelength of 506 nm; (2) having a molar absorption coefficient at 494 nm of 94600 M-1~cm-1~; (3) having a quantum yield of 0.65; and (4) having a low pH-sensitivity of the fluorescent properties in a pH range of 4 to 11.

L'invention concerne une protéine fluorescente présentant une nouvelle structure primaire issue d'un organisme autre que Aequorea coerulescens. L'invention concerne en particulier une protéine fluorescente issue de Halcurias sp. L, présentant les propriétés suivantes: (1) une longueur d'onde d'excitation maximum de 494 nm et une longueur d'onde de fluorescence maximum de 506 nm; (2) un coefficient d'absorption molaire de 94600 M-1cm-1 à 494 nm; (3) un rendement quantique de 0,65; et (4) des propriétés de fluorescence à faible sensibilité au pH sur une gamme de pH comprise entre 4 et 11.

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