Fluorescent proteins and methods of use thereof

Details Fluorescent proteins and methods of use thereof Fluorescent proteins and methods of use thereof

C - Chemistry, Metallurgy

12

N

C12N 15/00 (2006.01) C07K 1/00 (2006.01) C07K 14/435 (2006.01)

Patent

CA 2688521

The invention relates to dimeric and monomeric mutants of the naturally occurring tetrameric Anemonia sulcata Green Fluorescent Protein 499 (AsGFP499), peptides thereof and polynucleotides encoding them, and to their use as intracellular fluorescent markers particularly in the set up of cell-based or cell-free assays.

La présente invention concerne des mutants dimères et monomères provenant de la protéine 499 à fluorescence verte d'origine naturelle (AsGFP499) ou Anemonia sulcata, ses peptides et les polynucléotides les codant, ainsi que leur utilisation en tant que marqueurs fluorescents intracellulaires notamment lors d'analyses à base de cellules ou dépourvues de cellules.

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